Waterborne Tebuconazole Exposure Induces Male-Biased Sex Differentiation in Zebrafish (Danio rerio) Larvae via Aromatase Inhibition
文献类型： 外文期刊
作者： Qiao, Kun 1 ; Liang, Zhuoying 1 ; Wang, Aoxue 1 ; Wu, Qiong 1 ; Yang, Siyu 1 ; Ma, Yongfang 1 ; Li, Shuying 1 ; Schiwy, Sabrina 3 ; Jiang, Jinhua 6 ; Zhou, Shengli 7 ; Ye, Qingfu 2 ; Hollert, Henner 3 ; Gui, Wenjun 1 ;
作者机构： 1.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Peoples R China
2.Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310058, Peoples R China
3.Goethe Univ Frankfurt, Fac Biol Sci, Dept Evolutionary Ecol & Environm Toxicol, D-60438 Frankfurt, Germany
4.Patent Examinat Cooperat Henan, Ctr Patent Off, Zhengzhou 450046, Peoples R China
5.Zhejiang Univ, Key Lab Biol Crop Pathogens & Insects Zhejiang Pro, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insect Pests, Hangzhou 310058, Peoples R China
6.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou 310021, Peoples R China
7.Zhejiang Prov Environm Monitoring Ctr, Hangzhou 310012, Peoples R China
8.Fraunhofer Inst Mol Biol & Appl Ecol IME, Dept Environm Media Related Ecotoxicol, D-57392 Schmallenberg, Germany
9.LOEWE Ctr Translat Biodivers Genom LOEWE TBG, D-60325 Frankfurt, Germany
关键词： tebuconazole; zebrafish; sex differentiation; masculinization; aromatase
期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY （ 影响因子：11.4； 五年影响因子：12.0 ）
ISSN： 0013-936X
年卷期： 2023 年 57 卷 44 期
页码：
收录情况： SCI
摘要： Tebuconazole is a widely used fungicide for various crops that targets sterol 14-alpha-demethylase (CYP51) in fungi. However, attention has shifted to aromatase (CYP19) due to limited research indicating its reproductive impact on aquatic organisms. Herein, zebrafish were exposed to 0.5 mg/L tebuconazole at different developmental stages. The proportion of males increased significantly after long-term exposure during the sex differentiation phase (0-60, 5-60, and 19-60 days postfertilization (dpf)). Testosterone levels increased and 17 beta-estradiol and cyp19a1a expression levels decreased during the 5-60 dpf exposure, while the sex ratio was equally distributed on coexposure with 50 ng/L 17 beta-estradiol. Chemically activated luciferase gene expression bioassays determined that the male-biased sex differentiation was not caused by tebuconazole directly binding to sex hormone receptors. Protein expression and phosphorylation levels were specifically altered in the vascular endothelial growth factor signaling pathway despite excluding the possibility of tebuconazole directly interacting with kinases. Aromatase was selected for potential target analysis. Molecular docking and aromatase activity assays demonstrated the interactions between tebuconazole and aromatase, highlighting that tebuconazole poses a threat to fish populations by inducing a gender imbalance.
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