Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants
文献类型： 外文期刊
作者： Li, Jing 1 ; Xiang, Cong-Ying 1 ; Yang, Jian 1 ; Chen, Jian-Ping 1 ; Zhang, Heng-Mu 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Biotechnol Plant Protect MOA & Zhejiang P, Hangzhou 310021, Zhejiang, Peoples R China
2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004,
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2015 年 5 卷
页码：
收录情况： SCI
摘要： Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal cells of Nicotiana benthamiana. This is the first report that a plant virus or a viral protein alters the expression pattern or sub-cellular distribution of sHSPs.
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