P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts
文献类型： 外文期刊
作者： Liu, Xiao-Ya 1 ; Yang, Jian 2 ; Xie, Li 2 ; Li, Jing 2 ; Song, Xi-Jiao 3 ; Chen, Jian-Ping; Zhang, Heng-Mu;
作者机构： 1.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Plant Virol, Key Lab Plant Protect & Biotechnol,Minist Agr,Ins, Hangzhou 310021, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Plant Virol, Key Lab Plant Protect & Biot
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2015 年 160 卷 5 期
页码：
收录情况： SCI
摘要： The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
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