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Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans

文献类型： 外文期刊

作者： Cheng, Jianhui 1 ; Wang, Hu 3 ; Xu, Xin 2 ; Wang, Liangyan 2 ; Tian, Bing 2 ; Hua, Yuejin 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China

3.Hangzhou Normal Univ, Inst Ageing Res, Hangzhou, Zhejiang, Peoples R China

关键词： Deinococcus radiodurans;dr1790 disruptant;characteristics;functional analysis

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2015 年 46 卷 2 期

页码：

收录情况： SCI

摘要： Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.

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