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In silico identification and analysis of phytoene synthase genes in plants

文献类型: 外文期刊

作者: Han, Y. 1 ; Zheng, Q. S. 2 ; Wei, Y. P. 3 ; Chen, J. 1 ; Liu, R. 2 ; Wan, H. J. 4 ;

作者机构: 1.Suzhou Polytechn Inst Agr, Suzhou, Peoples R China

2.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Zhejiang, Peoples R China

关键词: Carotenoids;Evolution;Phytoene synthase

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 3 期

页码:

收录情况: SCI

摘要: In this study, we examined phytoene synthetase (PSY), the first key limiting enzyme in the synthesis of carotenoids and catalyzing the formation of geranylgeranyl pyrophosphate in terpenoid biosynthesis. We used known amino acid sequences of the PSY gene in tomato plants to conduct a genome-wide search and identify putative candidates in 34 sequenced plants. A total of 101 homologous genes were identified. Phylogenetic analysis revealed that PSY evolved independently in algae as well as monocotyledonous and dicotyledonous plants. Our results showed that the amino acid structures exhibited 5 motifs (motifs 1 to 5) in algae and those in higher plants were highly conserved. The PSY gene structures showed that the number of intron in algae varied widely, while the number of introns in higher plants was 4 to 5. Identification of PSY genes in plants and the analysis of the gene structure may provide a theoretical basis for studying evolutionary relationships in future analyses.

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