Transcriptome analysis of Acidovorax avenae subsp avenae cultivated in vivo and co-culture with Burkholderia seminalis
文献类型： 外文期刊
作者： Li, Bin 1 ; Ibrahim, Muhammad 1 ; Ge, Mengyu 1 ; Cui, Zhouqi 1 ; Sun, Guochang 2 ; Xu, Fei 3 ; Kube, Michael 4 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Detect Pesticide Residues, Minist Agr, Hangzhou 310021, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Digital Agr, Hangzhou 310021, Zhejiang, Peoples R China
4.Humboldt Univ, Fac Agr & Hort, D-14195 Berlin, Germany
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2014 年 4 卷
页码：
收录情况： SCI
摘要： Response of bacterial pathogen to environmental bacteria and its host is critical for understanding of microbial adaption and pathogenesis. Here, we used RNA-Seq to comprehensively and quantitatively assess the transcriptional response of Acidovorax avenae subsp. avenae strain RS-1 cultivated in vitro, in vivo and in co-culture with rice rhizobacterium Burkholderia seminalis R456. Results revealed a slight response to other bacteria, but a strong response to host. In particular, a large number of virulence associated genes encoding Type I to VI secretion systems, 118 putative non-coding RNAs, and 7 genomic islands (GIs) were differentially expressed in vivo based on comparative genomic and transcriptomic analyses. Furthermore, the loss of virulence for knockout mutants of 11 differentially expressed T6SS genes emphasized the importance of these genes in bacterial pathogenicity. In addition, the reliability of expression data obtained by RNA-Seq was supported by quantitative real-time PCR of the 25 selected T6SS genes. Overall, this study highlighted the role of differentially expressed genes in elucidating bacterial pathogenesis based on combined analysis of RNA-Seq data and knockout of T6SS genes.
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