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Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins

文献类型： 外文期刊

作者： Liu Yan 1 ; Qin Feng-yan 1 ; Bao Guo-lian 1 ; Chen Hui 1 ; Xiao Chen-wen 1 ; Wei Qiang 1 ; Ji Quan-an 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Anim Husb & Vet Inst, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Bordetella bronchiseptica;outer membrane proteins;immunoproteomic analysis

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 9 期

页码：

收录情况： SCI

摘要： Bordetella bronchiseptica is a Gram-negative pathogen that causes acute and chronic respiratory infection in a variety of animals. To identify useful antigen candidates for diagnosis and subunit vaccine of B. bronchiseptica, immunoproteomic analysis was adopted to analyse outer membrane proteins of it. The outer membrane proteins extracted from B. bronchiseptica were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the convalescent serum against two strains. Immunogenic proteins were identified by matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS), a total of 14 proteins are common immunoreactive proteins, of which 1 was known antigen and 13 were novel immunogenic proteins for B. bronchiseptica. Putative lipoprotein gene was cloned and recombinantly expressed. The recombinant protein induced high titer antibody, but showed low protective indices against challenges with HB (B. bronchiseptica strain isolated from a infected rabbit). The mortality of mice was 80% compared to 100% of positive controls. The identification of these novel antigenic proteins is an important resource for further development of a new diagnostic test and vaccine for B. bronchiseptica.

  • 相关文献

[1]Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins. Liu, Yan,Chen, Hui,Wei, Qiang,Xiao, Chenwen,Ji, Quanan,Bao, Guolian. 2015

[2]Greater efficacy of the ECMS-oil adjuvant over other formulations on immune responses against Bordetella bronchiseptica in rabbits and the underlying mechanism. Xiao, Chenwen,Bao, Guolian,Liu, Yan,Wei, Qiang,Ji, Quanan,Liu, Yaping,Pan, Lijun.

[3]Differential proteomics of outer membrane proteins between virulent and avirulent strains of Riemerella anatipestifer. Liu Yan,Wei Qiang,Bao Guo-Lian,Ji Quan-An. 2008

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