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Differential proteomics of outer membrane proteins between virulent and avirulent strains of Riemerella anatipestifer

文献类型： 外文期刊

作者： Liu Yan 1 ; Wei Qiang 1 ; Bao Guo-Lian 1 ; Ji Quan-An 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husbandry & Vet Med, Hangzhou 310021, Peoples R China

关键词： Riemerella anatipestifer;outer membrane proteins;two-dimensional polyacrylamide gel electrophoresis;virulence factor

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2008 年 35 卷 6 期

页码：

收录情况： SCI

摘要： In order to identify virulence factors of the pathogen, the outer membrane proteins of virulent and avirulent strains of Riemerella anatipestifer were compared by a proteome analysis. Three protein spots differentially expressed between the two strains were observed by 2-DE gels, and were further analyzed using in gel tryptic digestion and peptide mass fingerprinting. Three proteins were identified. W1 was Hsp20, W2 and W3 were transposon. Although the exact role of these proteins has not been characterized, the exclusive expression in virulent strain may indicate that they play an important role in the pathogenesis of Riemerella anatipestifer infection. Although only two virulence factors identified, it opens a path to the further analysis of virulence factors of Riemerella anatipestifer.

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[1]Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins. Liu Yan,Qin Feng-yan,Bao Guo-lian,Chen Hui,Xiao Chen-wen,Wei Qiang,Ji Quan-an. 2014

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