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Polysaccharide from Phellinus linteus induces S-phase arrest in HepG2 cells by decreasing calreticulin expression and activating the P27kip1-cyclin A/D1/E-CDK2 pathway

文献类型： 外文期刊

作者： Li, You-Gui 1 ; Ji, Dong-Feng 1 ; Zhong, Shi 1 ; Liu, Pei-Gang 1 ; Lv, Zhi-Qiang 1 ; Zhu, Jian-Xun 1 ; Chen, Jin-E 1 ; Ch 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

2.Hangzhou Qing Zheng Biol Sci Technol Co, Hangzhou 311700, Zhejiang, Peoples R China

关键词： Polysaccharide;S-phase arrest;Calreticulin;P27kip1;Cyclin/CDK complexes

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：4.36； 五年影响因子：4.488 ）

ISSN： 0378-8741

年卷期： 2013 年 150 卷 1 期

页码：

收录情况： SCI

摘要： Ethnopharmacology relevance: Our previous study showed that the proteoglycan P1 from Phellinus linteus (Mesima) exhibits significant anti-tumor activity against human hepatocellular carcinoma cells (HepG2); however, its molecular mechanism remains unknown. This study aims to provide insights into the mechanism of the anti-tumor activity of P1 against HepG2 cells. Methods: We examined the effects of P1 on HepG2 cell proliferation in vitro and in vivo. Flow cytometry was used to analyze the cell cycle distribution and apoptosis. Proteomic analysis, real-time (RT)-PCR, and Western blot were carried out to observe the expression of several cell cycle control proteins in HepG2 cells. Results: Both the volume and the weight of solid tumors were significantly decreased in P1-treated mice (200 mg/kg) compared with the control. The HepG2 cells in the P1-treated tumors were significantly decreased, irregularly shaped, and smaller. P1 slightly increased the body weight of the tumor-bearing mice, which indicates that P1 is nontoxic to mammals at 200 mg/kg. P1 also caused a significant dose-dependent increase in S phase arrest, but no apoptosis was observed in HepG2 cells. The results of the proteomic analysis, RT-PCR, and Western blot analysis showed that significantly downregulated expression of calreticulin, cyclin D1, cyclin E, and CDK2 and upregulated expression of P27kip1 and cyclin A in the P1-treated HepG2 cells (200 mu g/ml). Conclusion: These results suggest that calreticulin expression and the P27kip1-cydin A/D1/E-CDK2 pathway were involved in P1-induced S-phase cell cycle arrest in HepG2 cells. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

  • 相关文献

[1]Activation of P27kip1-cyclin D1/E-CDK2 pathway by polysaccharide from Phellinus linteus leads to S-phase arrest in HT-29 cells. Zhong, Shi,Ji, Dong-Feng,Li, You-Gui,Lin, Tian-Bao,Lv, Zhi-Qiang,Chen, Hua-Ping.

[2]Protocatechualdehyde Induces S-Phase Arrest and Apoptosis by Stimulating the p27(KIP1)-Cyclin A/D1-CDK2 and Mitochondrial Apoptotic Pathways in HT-29 Cells. Zhong, Shi,Li, You-Gui,Ji, Dong-Feng,Lin, Tian-Bao,Lv, Zhi-Qiang.

[3]Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method. Jin, Qun-li,Zhang, Zuo-fa,Lv, Guo-ying,Cai, Wei-ming,Fan, Lei-fa,Cheng, Jun-wen,Wang, Jian-gong.

[4]Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,He, Weiqiang,Shi, Liangen.

[5]Hypoglycemic effect of deoxynojirimycin-polysaccharide on high fat diet and streptozotocin-induced diabetic mice via regulation of hepatic glucose metabolism. Li, You-gui,Ji, Dong-feng,Zhong, Shi,Lin, Tian-bao,Lv, Zhi-qiang.

[6]Comparison of the preliminary characterizations and antioxidant properties of polysaccharides obtained from Phellinus baumii growth on different culture substrates. Zhang Zuo-fa,Lv Guo-ying,Song Ting-ting,Jin Qun-li,Fan Lei-fa,Cai Wei-ming,Huang Jian-bo.

[7]Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum. Zhang, Zuo-fa,Lv, Guo-ying,Cheng, Jian-hui,Fan, Lei-fa,Jiang, Xue.

[8]Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice. Li, You-Gui,Ji, Dong-Feng,Zhong, Shi,Lv, Zhi-Qiang,Lin, Tian-Bao,Chen, Shi,Hu, Gui-Yan. 2011

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