您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Shoot organogenesis and plant regeneration in Agave hybrid, No. 11648

文献类型： 外文期刊

作者： Zhang, Yan-Mei 1 ; Li, Xin 2 ; Chen, Zhi 3 ; Li, Jun-Feng 1 ; Lu, Jun-Ying 1 ; Zhou, Wen-Zhao 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Hainan Prov Key Lab Trop Crops Nutr, Zhanjiang 524091, Peoples R China

2.Hubei Inst Nationalities, Sch Biol Sci & Technol, Enshi 445000, Peoples R China

3.Zhejiang Acad Agr Sci, Virol & Biotechnol Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Agave hybrid No. 11648;Callus induction;Shoot organogenesis;Plant regeneration

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2013 年 161 卷

页码：

收录情况： SCI

摘要： The effects of explant type and the combination and concentration of plant growth regulators on callus induction and shoot regeneration in Agave hybrid No. 11648 were examined. Shoot tip and immature leaf segments were cultured on Murashige and Skoog (MS) medium supplied with different concentrations of benzyladenine (BA), at 0.2-3.0 mg l(-1), and alpha-naphthalene acetic acid (NAA), at 0.1-1.0 mg l(-1), or 2,4-dichlorophenoxyacetic acid (2,4-D), at 0.2-2.0 mg l(-1) for callus induction. The highest rate of light yellowgreen, compact and nodular-like calli was formed on the MS medium supplemented with 2.0 mg l(-1) BA in combination with 0.2 mg l(-1) NAA, with 93.2% of shoot tips producing a compact, light yellowgreen and nodular-like callus. The calli derived from the different explants were subcultured on Schenk and Hildebrandt (SH) medium (300 mg l(-1) ammonium nitrate, 2267 mg l(-1) potassium nitrate, 353 mg l(-1) potassium dihydrogen phosphate, 3 mg l(-1) glycine, 1.0 mg l(-1) thiamine hydrochloride (B-1), 1.0 mg l(-1) pyridoxine hydrochloride (B6), 1.0 mg l(-1) niacin, 200 mg l(-1) idositol) containing different concentrations of BA, NAA and indole-3-butyric acid (IBA) for shoot induction. Shoot organogenesis was achieved when the callus tissue derived from shoot tips was transferred to SH medium. Maximum organogenesis was obtained with 5.0 mg l(-1) BA in combination with 0.1 mg l(-1) NM and 0.1 mg l(-1) IBA, yielding 98.0% shoot induction frequencies. Regenerated shoots were rooted on growth regulator-free MS medium, with an average number of 5.39 roots per shoot, and a length of 8.44 cm per root within'30 days, and acclimatized in a greenhouse, with 98.57 1.43% survival. This is the first report of plant regeneration from Agave hybrid No. 11648 shoot tips. (C) 2013 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed. Hua, Wei,Zhu, Jinghuan,Shang, Yi,Wang, Junmei,Jia, Qiaojun,Lin, Feng,Yang, Jianming. 2013

[2]Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng,Xiaohui, Zhang,Gu, Honghui. 2011

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台