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Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation

文献类型： 外文期刊

作者： Yuan, Feng-Jie 3 ; Zhu, Dan-Hua 3 ; Tan, Yuan-Yuan 1 ; Dong, De-Kun 3 ; Fu, Xu-Jun 3 ; Zhu, Shen-Long 3 ; Li, Bai-Quan 4 ;

作者机构： 1.Zhejiang Univ, Key Lab Nucl Agr Sci, Minist Agr & Zhejiang Prov, Hangzhou 310029, Zhejiang, Peoples R China

2.Zhejiang Univ, IAEA Zhejiang Univ Collaborating Ctr, Inst Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Crop Sci & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Crop Sci & Nucl

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2012 年 125 卷 7 期

页码：

收录情况： SCI

摘要： Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6 hexakisphosphate) is an important constituent of soybean meal. Since phytic acid and its mineral salts (phytates) are almost indigestible for monogastrics, their abundance in grain food/feed causes nutritional and environmental problems; interest in breeding low phytic acid has therefore increased considerably. Based on gene mapping and the characteristics of inositol polyphosphates profile in the seeds of a soybean mutant line Gm-lpa-ZC-2, the soybean ortholog of inositol 1,3,4,5,6 pentakisphosphate (InsP(5)) 2-kinase (IPK1), which transforms InsP(5) into phytic acid, was first hypothesized as the candidate gene responsible for the low phytic acid alteration in Gm-lpa-ZC-2. One IPK1 ortholog (Glyma14g07880, GmIPK1) was then identified in the mapped region on chromosome 14. Sequencing revealed a G -> A point mutation in the genomic DNA sequence and the exclusion of the entire fifth exon in the cDNA sequence of GmIPK1 in Gm-lpa-ZC-2 compared with its wild-type progenitor Zhechun No. 3. The excluded exon encodes 37 amino acids that spread across two conserved IPK1 motifs. Furthermore, complete co-segregation of low phytic acid phenotype with the G -> A mutation was observed in the F-2 population of ZC-lpa x Zhexiandou No. 4 (a wild-type cultivar). Put together, the G -> A point mutation affected the pre-mRNA splicing and resulted in the exclusion of the fifth exon of GmIPK1 which is expected to disrupt the GmIPK1 functionality, leading to low phytic acid level in Gm-lpa-ZC-2. Gm-lpa-ZC-2, would be a good germplasm source in low phytic acid soybean breeding.

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