DEVELOPMENT AND CHARACTERIZATION OF 41 NOVEL EST-SSR MARKERS FOR PISUM SATIVUM (LEGUMINOSAE)
文献类型： 外文期刊
作者： Xu, Sheng-Chun 1 ; Gong, Ya-Ming 1 ; Mao, Wei-Hua 2 ; Hu, Qi-Zan 1 ; Zhang, Gu-Wen 1 ; Fu, Wan 2 ; Xian, Qiang-Qiang 2 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China
2.Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310029, Zhejiang, Peoples R China
3.Liaoning Normal Univ, Coll Life Sci, Dalian 116021, Peoples R China
关键词： expressed sequence tags;Leguminosae;microsatellites;pea;Pisum sativum;transferability
期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）
ISSN： 0002-9122
年卷期： 2012 年 99 卷 4 期
页码：
收录情况： SCI
摘要： Premise of the study: Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Pisum sativum for further use in genetic studies and breeding programs. Methods and Results: Forty-one novel EST-SSR primers were developed and characterized for size polymorphism in 32 Pisum sativum individuals from four populations from China. In each population, the number of alleles per locus ranged from one to seven, with observed heterozygosity and expected heterozygosity ranging from 0 to 0.8889 and 0 to 0.8400, respectively. Furthermore, 53.7% of these markers could be transferred to the related species, Vicia faba. Conclusions: The developed markers have potential for application in the study of genetic diversity, germplasm appraisal, and marker-assisted breeding in pea and other legume species.
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