Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells
文献类型: 外文期刊
作者: Zhou, Wen-Lin 1 ; Cao, Jin-Ru 1 ; Ye, Ai-Hong 1 ; Gong, Cheng-Liang 2 ; Weng, Hong-Biao 1 ; He, Li-Hua 1 ; Xue, Ren-Yu 2 ;
作者机构: 1.Zhejiang Acad Agr Sci, Sericulture Res Inst, Hangzhou 310021, Zhejiang, Peoples R China
2.Soochow Univ, Coll Preclin Med & Biol Sci, Suzhou 215123, Peoples R China
3.Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China
关键词: Escherichia coli heat-labile enterotoxin;piggyBac transposon;Bombyx mori;BmN cell;genetic transformation
期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )
ISSN: 1680-5593
年卷期: 2012 年 11 卷 20 期
页码:
收录情况: SCI
摘要: The non-toxic B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered antigens. To obtain transformed silkworm cell line stably expressing LTB, researchers fused the LTB coding sequence, neomycin-resistance gene (Neo(R)) and gfp gene into piggyBac-based fransponson vector and transduced into silkworm BmN cells. After screening against antibiotic G418, the positive rate of cells emitting green fluorescence was 70.79%. PCR detection indicates the existence of exogenous LTB coding sequence, Neo(R) and gfp in the transformed cell genome. Western blot analysis also confirms the predicated similar to 60 kDa band of LTB protein. These results demonstrated that the strategy was practicable.
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