文献类型： 外文期刊
作者： Xu, Xia 1 ; Wang, Yao-Hui 2 ; Liu, Zu-Lian 2 ; Wang, Yong-Qiang 1 ; He, Lin 3 ; Li, Kai 3 ; Huang, Yong-Ping 2 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Sericulture & Tea Res, Hangzhou 310021, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China
3.East China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China
关键词： Bombyx mori; CRISPR; Cas9; Esp; female sterility
期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）
ISSN： 1672-9609
年卷期：
页码：
收录情况： SCI
摘要： Yolk proteins are the main source of nutrients during embryonic and early larval development in oviparous animals. Therefore, vitellogenesis is crucial for reproduction. The silkworm, Bombyx mori, is a model lepidopteran insect in which there are three yolk proteins: vitellin, 30-kDa protein, and egg-specific protein (Esp). In this study, we explored the gene function of Esp through transgenic clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9 technology-mediated mutations in the silkworm. We found that Esp mutation resulted in female sterility but had no effect on male fertility. Female mutants could lay eggs after mating, but the eggs were smaller and lighter colored than those laid by wild-type females. The most important finding is that the eggs laid by female mutants did not hatch. Furthermore, we observed stable inheritance of female sterility caused by Esp mutation through successive generations. Thus, Esp encodes a yolk protein that is crucial for female reproductive success and is a potential target for pest control.
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