Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis)
文献类型： 外文期刊
作者： Sheng, Xiaoguang 1 ; Zhao, Zhenqing 1 ; Yu, Huifang 1 ; Wang, Jiansheng 1 ; Xiaohui, Zhang 1 ; Gu, Honghui 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Cauliflower;Protoplast;Plant regeneration;Ploidy level;SRAP analysis
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）
ISSN： 0167-6857
年卷期： 2011 年 107 卷 3 期
页码：
收录情况： SCI
摘要： Protoplasts were isolated from hypocotyls of 5-day-old seedlings of five doubled-haploid (DH) lines of cauliflower after enzymatic digestion in 0.1% macerozyme R-10 and 1.0% cellulose R-10. Shoot regeneration was achieved in four of the five DH lines. Protoplast yield and frequency of cell division and shoot regeneration varied among experiments and DH lines. Sequence-related amplified polymorphism (SRAP) analysis on all the regenerated plants of each DH line indicated that their genetic compositions were homologous with their mother plants, but few regenerated plants of DH lines no. 3 and 5 were detected with changes in ploidy levels.
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