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Evaluation of genetic diversity in self-incompatible broccoli DH lines assessed by SRAP markers

文献类型： 外文期刊

作者： Yu, Huifang 1 ; Zhao, Zhenqing 1 ; Sheng, Xiaoguang 1 ; Wang, Jiansheng 1 ; Gu, Honghui 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Brassica oleracea L. var. italica;self-compatibility index;double haploid;genetic diversity;sequence related amplified polymorphism (SRAP)

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 59 期

页码：

收录情况： SCI

摘要： In this article, we investigated self-compatibility index (SCI) in broccoli double haploid (DH) lines, and the relationship and genetic diversity of 15 self-incompatible (SI) broccoli DH lines were analyzed by sequence related amplified polymorphism (SRAP). 11 primer combinations selected from 88 primer pairs revealed a total number of 129 unambiguous bands, 61 of which were polymorphic with a polymorphism frequency of 47.3%. Analyzed by NTSYS software, the genetic similarity coefficient of the 15 broccoli resources ranged from 0.76 to 0.98. Based on the coefficient value of 0.79, these broccoli DH lines were clustered into three multiple-member groups by unweighted pair group method with arithmetic mean (UPGMA) analysis, which provided molecular reference for parent selection in broccoli breeding.

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