您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice

文献类型： 外文期刊

作者： Li, You-Gui 1 ; Ji, Dong-Feng 1 ; Zhong, Shi 1 ; Lv, Zhi-Qiang 1 ; Lin, Tian-Bao 1 ; Chen, Shi 1 ; Hu, Gui-Yan 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

关键词： 1-Deoxynojirimycin;Polysaccharide;Mulberry leaves;Diabetes mellitus

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：4.36； 五年影响因子：4.488 ）

ISSN： 0378-8741

年卷期： 2011 年 134 卷 3 期

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: 1-Deoxynojirimycin (DNJ) discovered from mulberry trees has been reported to be a potent inhibitor of intestinal alpha-glycosidases (sucrase, maltase, glucoamylase), and many polysaccharides were useful in protecting against alloxan-induced pancreatic islets damage through their scavenging ability. This study was aimed to evaluate the therapeutic effect and potential mechanism(s) of the hybrid of DNJ and polysaccharide (HDP) from mulberry leaves on alloxan-induced diabetic mice. Materials and methods: Daily oral treatment with HDP (150 mg/kg body weight) to diabetic mice for 12 weeks, body weight and blood glucose were determined every week, oral glucose tolerance test was performed after 4 and 8 weeks, biochemical values were measured using assay kits and gene expressions were investigated by RT-PCR. Results: A significant decline in blood glucose, glycosylated hemoglobin, triglyceride, aspartate transaminase and alanine transaminase levels and an evident increase in body weight, plasma insulin level and high density lipoprotein were observed in HDP treated diabetic mice. The polysaccharide (P1) showed a significant scavenging hydroxyl radicals and superoxide anion radical effects in vitro, which indicated that P1 could protect alloxan-induced pancreatic islets from damage by scavenging the free radicals and repaired the destroyed pancreatic beta-cells. Pharmacokinetics assay showed that DNJ could be absorbed from the gastrointestinal mucosa and diffused rapidly into the liver, resulted in postprandial blood glucose decrease and alleviated the toxicity caused by sustained supra-physiological glucose to pancreatic beta-cells. RT-PCR results indicated that HDP could modulate the hepatic glucose metabolism and gluconeogenesis by up/down-regulating the expression of rate-limiting enzymes (glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) in liver and up-regulating the pancreatic and duodenal homeobox factor-1 (PDX-1), insulin-1 and insulin-2 expressions in pancreas. Conclusion: These findings suggested that HDP has complimentary potency to develop an antihyperglycemic agent for treatment of diabetes mellitus. Crown Copyright (C) 2011 Published by Elsevier Ireland Ltd. All rights reserved.

  • 相关文献

[1]Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method. Jin, Qun-li,Zhang, Zuo-fa,Lv, Guo-ying,Cai, Wei-ming,Fan, Lei-fa,Cheng, Jun-wen,Wang, Jian-gong.

[2]Activation of P27kip1-cyclin D1/E-CDK2 pathway by polysaccharide from Phellinus linteus leads to S-phase arrest in HT-29 cells. Zhong, Shi,Ji, Dong-Feng,Li, You-Gui,Lin, Tian-Bao,Lv, Zhi-Qiang,Chen, Hua-Ping.

[3]Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,He, Weiqiang,Shi, Liangen.

[4]Hypoglycemic effect of deoxynojirimycin-polysaccharide on high fat diet and streptozotocin-induced diabetic mice via regulation of hepatic glucose metabolism. Li, You-gui,Ji, Dong-feng,Zhong, Shi,Lin, Tian-bao,Lv, Zhi-qiang.

[5]Comparison of the preliminary characterizations and antioxidant properties of polysaccharides obtained from Phellinus baumii growth on different culture substrates. Zhang Zuo-fa,Lv Guo-ying,Song Ting-ting,Jin Qun-li,Fan Lei-fa,Cai Wei-ming,Huang Jian-bo.

[6]Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum. Zhang, Zuo-fa,Lv, Guo-ying,Cheng, Jian-hui,Fan, Lei-fa,Jiang, Xue.

[7]Polysaccharide from Phellinus linteus induces S-phase arrest in HepG2 cells by decreasing calreticulin expression and activating the P27kip1-cyclin A/D1/E-CDK2 pathway. Li, You-Gui,Ji, Dong-Feng,Zhong, Shi,Liu, Pei-Gang,Lv, Zhi-Qiang,Zhu, Jian-Xun,Chen, Jin-E,Chen, Hua-Ping. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台