文献类型： 外文期刊

作者： Jiang, Chenglin ¹ ; Sun, Ju ¹ ; Li, Rui ¹ ; Yan, Shijuan ² ; Chen, Wei ¹ ; Guo, Liang ¹ ; Qin, Guochen ³ ; Wang, Pengcheng ³ ; Luo, Cheng ¹ ; Huang, Wenjie ² ; Zhang, Qinghua ¹ ; Fernie, Alisdair R. ⁴ ; Jackson, David ¹ ; Li, Xiang ⁶ ; Yan, Jianbing ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preser, Guangzhou 510640, Peoples R China

3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China

4.Max Planck Inst Mol Plant Physiol, Dept Mol Physiol, Muhlenberg 1, D-14476 Potsdam, Germany

5.Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

6.Chinese Acad Sci, Innovat Acad Seed Design, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

7.Chinese Acad Sci, Innovat Acad Seed Design, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

8.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

关键词： haploid induction; single-cell sequencing; maize; ROS; peroxidase

期刊名称：MOLECULAR PLANT （ 影响因子：21.949； 五年影响因子：19.617 ）

ISSN： 1674-2052

年卷期： 2022 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Haploid induction (HI) is an important tool in crop breeding. Phospholipase A1 (ZmPLA1)/NOT LIKE DAD (NLD)/MATRILINEAL (MTL) is a key gene controlling HI in maize; however, the underlying molecular mech-anism remains unclear. In this study, to dissect why loss of ZmPLA1 function could mediate HI we per -formed a comprehensive multiple omics analysis of zmpla1 mutant anthers by integrating transcriptome, metabolome, quantitative proteome, and protein modification data. Functional classes of significantly en-riched or differentially abundant molecular entities were found to be associated with the oxidative stress response, suggesting that a reactive oxygen species (ROS) burst plays a critical role in HI. In support of this, we further discovered that a simple chemical treatment of pollen with ROS reagents could lead to HI. Moreover, we identified ZmPOD65, which encodes a sperm-specific peroxidase, as a new gene control-ling HI. Taken together, our study revealed a likely mechanism of HI, discovered a new gene controlling HI, and created a new method for HI in maize, indicating the importance of ROS balance in maintaining normal reproduction and providing a potential route to accelerate crop breeding.