浙江省农业科学院机构知识库

Mapping the self-interacting domains of TuMV HC-Pro and the subcellular localization of the protein

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文献类型：外文期刊

作者： Zheng, Hongying ¹ ; Yan, Fei ¹ ; Lu, Yuwen ¹ ; Sun, Liying ¹ ; Lin, Lin ¹ ; Cai, Li ² ; Hou, Mingsheng ² ; Chen, Jianping ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

关键词： Turnip mosaic virus;HC-Pro;Subcellular localization;Interaction;YTH;BiFC

期刊名称：VIRUS GENES （影响因子：2.332；五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2011 年 42 卷 1 期

页码：

收录情况： SCI

摘要： The helper component-proteinase (HC-Pro) of potyviruses is a multifunctional protein involved in aphid transmission, polyprotein processing, cell-to-cell and long-distance movement, genome amplification and symptom expression. The HC-Pros of several potyviruses interact with themselves but the key domains responsible for self-interaction are apparently not conserved. In our experiments, yeast two-hybrid assays and bimolecular fluorescence complementation showed that Turnip mosaic virus (TuMV) HC-Pro interacted with itself in yeast cells, plant cells and insect cells. It was also shown that the central and C-terminal regions of the HC-Pro participated in these self-interactions. Fluorescence microscopy showed that TuMV HC-Pro was present in the cytoplasm and formed aggregates along the ER.

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