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Preparation and characterization of alpha-galactosidase-loaded chitosan nanoparticles for use in foods

文献类型: 外文期刊

作者: Liu, Yong 1 ; Sun, Yan 2 ; Li, Yanli 1 ; Xu, Shaochun 1 ; Tang, Jiangwu 1 ; Ding, Juntao 2 ; Xu, Yaoxing 1 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China

2.Hangzhou Normal Univ, Hangzhou 310012, Zhejiang, Peoples R China

关键词: alpha-Galactosidase;Chitosan;Nanoparticles;Encapsulation

期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )

ISSN: 0144-8617

年卷期: 2011 年 83 卷 3 期

页码:

收录情况: SCI

摘要: The instability of enzymes used in animal and human foods has long been an important issue in food preparation and storage. Recent breakthroughs in nanotechnology have shown the great advantages of nanoparticles (NPs) in protein encapsulation and controlled release. alpha-Galactosidase and chitosan (CS) were chosen as models for food enzymes and coating materials, respectively. A series of alpha-galactosidase-loaded CS NPs were prepared. The burst release of alpha-galactosidase from NPs and thermal stability of alpha-galactosidase after coating were tested. alpha-Galactosidase-loaded NPs were characterized by Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), transmission electron microscopy (TEM), photon correlation spectroscopy (PCS), and zeta potential analysis. The results were as follows: (1) alpha-Galactosidase was successfully encapsulated in CS NPs. (2) alpha-Galactosidase-loaded CS NPs had high encapsulation efficiency. (3) The thermal stability of alpha-galactosidase was significantly increased after loading with CS NPs. (4) The burst release efficiency of CS NPs loaded with alpha-galactosidase was higher than 90% at pH 3.0 and 10.0. (C) 2010 Elsevier Ltd. All rights reserved.

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