文献类型： 外文期刊

作者： Wu, Y. J. ¹ ; Xie, M. ¹ ; Long, Q. J. ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China

关键词： Actinidia eriantha Benth;kiwifruit;organogenesis;in vitro;leaf blade strips;petiole;wet moss;regeneration

期刊名称：NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE （ 影响因子：1.154； 五年影响因子：1.424 ）

ISSN： 0114-0671

年卷期： 2011 年 39 卷 4 期

页码：

收录情况： SCI

摘要： Plantlets were regenerated from leaf explants of Actinidia eriantha Benth. cv White (kiwifruit). The best medium for adventitious shoot formation from leaf blade strips was Murashige-Skoog (MS) medium with 0.1 mg L(-1) NAA and 2.0 mg L(-1) BA. The frequency of shoot regeneration (%) was dependent on the size and the part of the leaf from which the explant was taken. The highest frequency of shoot regeneration was 7.30 +/- 2.61 shoots per explant, for explants taken from within 0-3 cm of the leaf blade base, near a vein and not on the leaf edge. When using petiole-derived explants, a maximum frequency of adventitious shoot regeneration of 79.17% with 6.54 +/- 2.31 shoots per explant was obtained using 0.1 mg L(-1) NAA and 2.0 mg L(-1) ZT in MS medium. The highest rate of rooting obtained was 96.67% when using 1/2 MS medium containing 0.6 mg L(-1) IBA and 93.75% when using wet moss without IBA. This is the first report to study the effect of explants taken from different parts of the leaf blade on shoot regeneration in A. eriantha. All plantlets were transferred to pots of peat/perlite and grown on before being planted in the field. Plantlets were successfully acclimatized to field conditions and produced healthy plants.