您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Recent progress on technologies and applications of transgenic poultry

文献类型： 外文期刊

作者： Li, Jin-Jun 1 ; Lu, Li-Zhi 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Lab Poultry Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Genetic modification;embryo;blastodermal cells;primordial germ cells;stem cells;viral vector

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2010 年 9 卷 24 期

页码：

收录情况： SCI

摘要： Manipulation of the poultry genome has the potential to improve poultry production and offer a powerful bioreactor for the production of pharmaceutical and industrial proteins in eggs. However, before realizing this, the methods for producing transgenic poultry must become routine. The direct modification of an embryo with DNA or viral vectors is possible, but this approach does not have the ability to make locus-specific modifications to the genome. To overcome this problem, several cell-based protocols, which use mainly blastodermal cells (BDCs), embryonic stem cells (ESCs), primordial germ cells (PGCs) and spermatogonial stem cells (SSCs) have been developed to generate transgenic chickens. At present, the complete system for isolating, expanding, transfecting, selecting and re-expanding embryonic stem cell cultures and the subsequent production of high-grade somatic chimeras has been reported, but germline chimeras with transgenic progeny have not yet been achieved. Although the use of viral systems can achieve highly efficient gene transfer, the potential safety issues may limit their practical application. Among the many possible permutations and combinations of target cell and gene transfer methods described in this review, targeting SSCs in vitro using non-viral-based gene transfer and re-injecting them to cock testis is the most efficient and cost-effective strategy to produce transgenic poultry.

  • 相关文献

[1]MBD1 and MeCP2 expression in embryos and placentas from transgenic cloned goats. Jia, Ruoxin,Zhang, Guomin,Fan, Yixuan,Zhou, Zhengrong,Wan, Yongjie,Wang, Ziyu,Wang, Feng,Jia, Ruoxin,Jia, Ruoxin,Zhang, Guomin,Fan, Yixuan,Zhou, Zhengrong,Wan, Yongjie,Zhang, Yanli,Wang, Ziyu,Wang, Feng.

[2]Control of Rice Embryo Development, Shoot Apical Meristem Maintenance, and Grain Yield by a Novel Cytochrome P450. Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Yang, Weibing,Gao, Mingjun,Yin, Xin,Zeng, Longjun,Li, Qun,He, Zuhua,Liu, Jiyun,Wang, Junmin,Zhang, Xiaoming,Xu, Yonghan,Zhang, Shubiao.

[3]Proteome analysis on lethal effect of l (2) in the sex-linked balanced lethal strains of silkworm, Bombyx mori. Chen, Jine,Zhong, Boxiong,Chen, Jine,Niu, Baolong,Wang, Yongqiang,Liu, Yan,Liu, Peigang,Meng, Zhiqi. 2012

[4]Developmental increases in hypothalamic neuropeptide Y content with the embryonic age of meat- and layer-type chicks. Zhou, WD,Aoyama, M,Yoshizawa, F,Sugahara, K. 2006

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台