Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase
文献类型: 外文期刊
作者: Li, Yin 1 ; Liu, Zhiqiang 2 ; Cui, Fengjie 4 ; Ping, Lifeng 5 ; Qiu, Chongyan 6 ; Li, Geng 3 ; Yan, Lijiao 6 ;
作者机构: 1.N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA
2.Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China
3.New York Univ, Metro Tech Ctr 6, Polytech Inst, NSF I UCRC Biocatalysis & Bioproc Macromol, Brooklyn, NY 11201 USA
4.Jiangsu Univ, Sch Food & Biol Engn, Jiangsu 2120013, Zhenjiang, Peoples R China
5.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Peoples R China
6.Chongqing Inst Technol, Sch Biotechnol, Chongqing 400050, Peoples R China
7.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China
关键词: Alternaria sp;ND-16;Isolation;Identification;Production;Xylanase
期刊名称:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ( 影响因子:2.926; 五年影响因子:2.685 )
ISSN: 0273-2289
年卷期: 2009 年 157 卷 1 期
页码:
收录情况: SCI
摘要: Alternaria sp. ND-16, a bacterium isolated from soil sample, was identified as a strain of Alternaria mali based on the morphology and comparison of internal transcribed spacer rDNA gene sequence studies. Furthermore, it is demonstrated that this strain has xylanase activity, and the activity can be optimized under suitable growing conditions where wheat bran and urea are the primary sources of carbon and nitrogen. Partially purified xylanase from Alternaria sp. ND-16 is shown to have an optimal pH of 6.0 and optimal temperature of 50 A degrees C, making this enzyme potentially suitable for industrial applications. It is also demonstrated that Na(+) and Mn(2+) show strong inhibition of the xylanase while K(+), Li(+), Fe(2+), Cu(2+), and Zn(2+) have no significant effect on the activity.
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