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Genetic diversity of the VP1 gene of duck hepatitis virus type I (DHV-I) isolates from southeast China is related to isolate attenuation

文献类型： 外文期刊

作者： Liu, Guangqing 1 ; Wang, Fei 2 ; Ni, Zheng 1 ; Yun, Tao 1 ; Yu, Bin 1 ; Huang, Jionggang 1 ; Chen, Jianping 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

2.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Anhui, Peoples R China

关键词： duck hepatitis virus type I;DHV-I;phylogenetic tree;VP1

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2008 年 137 卷 1 期

页码：

收录情况： SCI

摘要： The complete sequence of an isolate (ZJ-V) of Duck hepatitis virus I (DHV-I), originally taken front the field in southeast China was determined. It was 7691 nucleotides long and had 5'- and 3'-terminal non-coding regions of 626 and 315 nucleotides, respectively. The poly(A) tail contained at least 22 residues and the single open reading frame encoded a polypeptide of 2249 amino acids. The VP1 gene was also sequenced from nine southeast China field isolates and three attenuated DHV-I vaccine strains. in phylogenetic analysis of the isolates and other published sequences, attenuated and tissue-adapted isolates (including ZJ-V) clustered as genotypes significantly different from the field isolates that had not been passaged in chicken/duck embryos. There were two consistent amino acid substitutions (E-129 -> V-129 and A(142) -> S-142) between all the field isolates and all the tissue-adapted ones. The carboxyl terminal region was generally the most variable and here the four attenuated Chinese isolates showed six consistent differences from the field isolates (S-181 -> L-181, H183K184 -> R(183)G(1841), N-193 -> D-193, F-205 -> K-205, R-217 -> K-217, N-235 -> D-235). It seems likely that at least some of these differences result from mutations leading to isolate attenuation. (C) 2008 Elsevier B.V. All rights reserved.

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