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Isolation and identification of a novel Rhodococcus sp ML-0004 producing epoxide hydrolase and optimization of enzyme production

文献类型： 外文期刊

作者： Liu, Zhiqiang 1 ; Li, Yin 2 ; Ping, Lifeng 2 ; Xu, Yingying 2 ; Cui, Fengjie 3 ; Xue, Yaping; Zheng, Yuguo;

作者机构： 1.Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Peoples R China

2.Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Peoples R China; N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA; Zhejiang Acad Agr Sci, Inst Qual & Standard Agr Prod, Hangzhou 310021, Peoples R China; Jiangsu Univ, Sch Food Engn & Biotechnol, Zhenjiang 2120013, Peoples R China

3.Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Peoples R Chin

关键词： Rhodococcus opacus ML-0004;epoxide hydrolase;L.(+)-tartaric acid;isolation;identification

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2007 年 42 卷 5 期

页码：

收录情况： SCI

摘要： Rhodococcus sp. ML-0004, a novel strain for producing epoxide hydrolase, was isolated from soil in this study. The epoxide hydrolase can catalyze the stereo-specific hydrolysis of cis-epoxysuccinic acid to generate L(+)-tartaric acid. By examining physiological, biochemical characteristics and comparing its 16S rDNA gene sequence, it was identified as Rhodococcus opacus, and named R. opacus ML-0004. The optimal conditions for epoxide hydrolase production from R. opacus ML-0004 were also investigated. Propanediol and (NH4)(2)SO4 were selected as carbon source and nitrogen source, respectively, for the production of R. opacus ML-0004 epoxide hydrolase. The optimal conditions for epoxide hydrolase production were fermentation temperature = 28 degrees C, pH 7.0, and cultivation time = 26 h. Under these conditions, the maximum epoxide hydrolase activity reached 10.5 U mL(-1). (C) 2007 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme. Liu, Zhiqiang,Li, Yin,Xu, Yingying,Ping, Lifeng,Zheng, Yuguo.

[2]Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase. Li, Yin,Liu, Zhiqiang,Liu, Zhiqiang,Li, Geng,Cui, Fengjie,Ping, Lifeng,Qiu, Chongyan,Yan, Lijiao,Yan, Lijiao. 2009

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