文献类型： 外文期刊

作者： Zhu, H. Q. ¹ ; Chen, J. P. ² ; Yu, S. Q. ² ;

作者机构： 1.Zhejiang Acad Agr Sci, Virol & Biotechnol Inst, Hangzhou, Peoples R China

2.Zhejiang Acad Agr Sci, Virol & Biotechnol Inst, Hangzhou, Peoples R China; Fudan Univ, Dept Microbiol, Shanghai 200433, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2006 年 151 卷 9 期

页码：

收录情况： SCI

摘要： Infectious cDNA clones of the Shanghai isolate of Ribgrass mosaic virus (RMV) were produced by joining four overlapping cDNA fragments and also in a single step by long template PCR. After inoculation of Nicotiana glutinosa with either RNA transcripts or the cDNA under the control of the CaMV 35S promoter, plants developed typical symptoms, and viral coat protein could be detected in them by Western blot analysis. However, compared to plants inoculated with purified viral RNA, lesions were fewer and appeared more slowly. An epitope of the Mycobacterium tuberculosis 31-kDa protein was inserted at the C-terminus of the viral coat protein by PCR using two overlapping fragments. The modified clone was also infectious and the foreign epitope could be detected serologically in the electron microscope and by Western blot analysis. The results demonstrate the potential of RMV as a viral gene vector.