Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision
文献类型: 外文期刊
作者: Zhang, GQ 1 ; Zhang, DQ 2 ; Tang, GX 2 ; He, Y 2 ; Zhou, WJ 2 ;
作者机构: 1.Zhejiang Univ, Coll Agr & Biotechnol, Inst Crop Sci, Hangzhou 310029, Peoples R China
2.Zhejiang Univ, Coll Agr & Biotechnol, Inst Crop Sci, Hangzhou 310029, Peoples R China; Zhejiang Acad Agr Sci, Inst Crop Sci, Hangzhou 310021, Peoples R China
关键词: auxin;Brassica napus L.;cold treatment;cytokinin;embryogenesis;gibberellin;successive subculture
期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )
ISSN: 0006-3134
年卷期: 2006 年 50 卷 2 期
页码:
收录情况: SCI
摘要: The present study evaluated the effects of chilling, partial desiccation, cotyledon excision and successive subculture of microspore-derived embryos on plant development in oilseed rape (Brassica napus L.). The results showed that out of the five media, all the genotypes showed the best response when the embryos were cultured on the half-strength Murashige and Skoog medium with 2.0 mg dm(-3) benzylaminopurine. A cold treatment for 3 or 5 d further increased frequencies of embryo germination (90.0 %) and plantlet development (58.46 %). Desiccation for one day also increased the embryo germination and plantlet development in all genotypes tested. Cutting the cotyledons of the embryos at late cotyledonary stage significantly increased the frequency of plantlet development. The highest rate of plantlet development was obtained from cultures of embryos sampled with size of less than 4.0 mm. The successive subculture further improved the germination and development of plantlets from embryos. In the genotype ZJU452, the rate of plantlet development reached 99.78 % after the second subculture of embryos.
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