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Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein readthrough and 19 ku cysteine-rich domains and localization of these proteins

文献类型： 外文期刊

作者： Xu, L 1 ; Chen, JP 2 ; Ye, R 2 ; Zhao, XY 2 ; Yu, SQ 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Virol Lab, Shanghai 200433, Peoples R China

2.Zhejiang Acad Agr Sci, Virol Lab, Shanghai 200433, Peoples R China; Fudan Univ, Dept Microbiol, Shanghai 200433, Peoples R China

关键词： Chinese wheat mosaic virus;coat protein readthrough domain;cysteine-rich domain;prokaryotic expression;localisation

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2002 年 47 卷 12 期

页码：

收录情况： SCI

摘要： The 5-terminal (RTn) and 3'-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coli. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins In CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was In the cytoplasm of the Infected wheat cells.

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