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Cloning and sequencing of the genome segment s9 of rice black-streaked dwarf virus

文献类型： 外文期刊

作者： Zhang, HM 1 ; Chen, JP 2 ; Cheng, Y 2 ; Lei, JL 2 ; Xue, QZ 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China; Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310029, Peoples R China

关键词： RBSDV;genome segment S9;sequence analysis

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 2001 年 33 卷 4 期

页码：

收录情况： SCI

摘要： Genome segment S9's of rice black-streaked dwarf virus (RBSDV) of three Chinese isolates were amplified by RT-PCR and sequenced, and were found to be consisted of 1 900 nt (RBSDV-Zj S9 EMBL accession number: AJ297430), 1 898 nt ( RBSDV-Heb S9 EMBL accession number: AJ297429) and 1 900 nt (RBSDV-Hub S9 EMBL accession number. AJ291706), respectively. Genome segment S9's of three Chinese Isolates shared 98.5%-98.8% sequence homology and all contained two open reading frames (ORF), which encoded two polypeptides with molecular weights of 40 kD and 24 kD, respectively. Amino acid sequence comparison of the polypeptides encoded by the second ORF of the corresponding genomic segment of five isolates, including three Chinese isolates, Japanese isolate, and Italian MRDV (maize rough dwarf virus), were highly conserved.

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