A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family
文献类型： 外文期刊
作者： Chen, J 1 ; Chen, J 1 ; Adams, MJ 3 ;
作者机构： 1.Zhejiang Acad Agr Sci, Dept Virol, Hangzhou 310021, Peoples R China
2.Zhejiang Univ, Coll Life Sci, Hangzhou 310027, Peoples R China
3.IACR Rothamsted, Dept Plant Pathol, Harpenden, Herts, England
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2001 年 146 卷 4 期
页码：
收录情况： SCI
摘要： A universal primer (Sprimer: 5'-GGX AAY AAY AGY GGX CAZ CC-3', X = A, G, C or T; Y = T or C; Z = A or G), designed from the consensus sequences that code for the conserved sequence GNNSGQP in the NIb region of members of the family Potyviridae, was used to amplify by RT-PCR the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. Sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (ATTC PV-107) of Oat necrotic mottle virus is not a distinct species in the genus Rymovirus, but is synonymous with Brome streak mosaic vir us (genus Tritimovirus) and that Celery mosaic virus is a distinct member of the genus Potyvirus not closely related to any other sequenced species. Potyviruses infecting crops in China were also investigated, showing that viruses on cowpea and maize in Hangzhou, Zhejiang province were respectively Bean common mosaic vints and Sugarcane mosaic virus and that one on garlic in Nanjing, Jiangsu province was Onion yellow dwarf virus. Fragments were also sequenced from Chinese isolates of Lettuce mosaic virus and Soybean mosaic virus (from Hangzhou), Turnip mosaic virus (2 different isolates from Zhejiang province) and RNA1 of Wheat yellow, mosaic virus (from Rongcheng, Shandong province).
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