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Anther culture in connection with induced mutations for rice improvement

文献类型: 外文期刊

作者: Chen, QF 1 ; Wang, CL 2 ; Lu, YM 2 ; Shen, M 2 ; Afza, R 2 ; Duren, MV 2 ; Brunner, H 2 ;

作者机构: 1.Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China; FAO, IAEA, Agr & Biotechnol Lab, Plant Breeding Unit, Seibersdorf, Austria

关键词: colchicine;doubled haploids;gamma rays;mutagenesis;mutants;Oryza sativa

期刊名称:EUPHYTICA ( 影响因子:1.895; 五年影响因子:2.181 )

ISSN: 0014-2336

年卷期: 2001 年 120 卷 3 期

页码:

收录情况: SCI

摘要: Doubled haploids have long been recognized as a valuable tool in plant breeding since it not only offers the quickest method of advancing heterozygous breeding lines to homozygosity, but also increases the selection efficiency over conventional procedures due to better discrimination between genotypes within any one generation. Ten cultivars of japonica rice and nine cultivars of indica rice were evaluated for androgenic response. Various doses (10-50 Gy) of gamma rays were applied to investigate the effect of radiation on callus formation, green plant regeneration and the frequency of selected doubled haploid mutants. Similarly, the effects of colchicine concentration (10-200 mg/l) on callus induction, regeneration and fertility of green plants were observed. It was demonstrated that the dose of 20 Gy gamma rays and 30 mg/l concentration of colchicine have significant stimulation effect on regeneration of green plants from rice anther culture. The high frequency of observed doubled haploid mutants indicates that anther culture applied in connection with gamma rays is an effective way to improve rice cultivars.

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