文献类型： 外文期刊
作者： Xie, JH 1 ; Zapata-Arias, FJ 2 ; Shen, M 2 ; Afza, R 2 ;
作者机构： 1.Int Atom Energy Agcy Labs, FAO IAEA Agr & Biotechnol Lab, Plant Breeding Unit, A-2444 Seibersdorf, Austria
2.Int Atom Energy Agcy Labs, FAO IAEA Agr & Biotechnol Lab, Plant Breeding Unit, A-2444 Seibersdorf, Austria; Zhejiang Agr Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China; Zhejiang Acad Agr Sci, Inst Applicat Atom Energy, Hangzhou 310021, Peoples R China
关键词： PCR;RAPDS;rice;salt-tolerance
期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）
ISSN： 0014-2336
年卷期： 2000 年 116 卷 2 期
页码：
收录情况： SCI
摘要： The genetic diversity of three salinity tolerant rice varieties Pokkali, Nona-Bokra and Bicol was investigated using random amplified polymorphic DNAs (RAPDs). Hi,oh yielding susceptible variety IR29 was used as check for comparison. The salinity performance of these varieties were tested by using rapid screening techniques at seedling stage. One hundred primers tested of which 42 revealed differences between Pokkali & Nonabokra, 43 between Pokkali st Bicol and 50 between Nonabokra and Bicol. Polymorphism differences between IR29 - Pokkali, IR29 - NonaBokra and IR29 - Bicol were 47%, 53% and 31%, respectively. Four primers amplified specific fragments that appeared in all the three salt tolerant varieties but not in the salt susceptible variety IR 29. Primer UBC 9 (5'-CCTGCGCTTA-3') produced a prominent diagnostic fragment of approximately 1600 bp; primer UBC 244 (5'-CAGCCAACCG-3') generated a fragment of about 800 bp in the salt-tolerant varieties; primer UBC 251 (5'-CTTGACGGGG-3') amplified one polymorphic band of 1100 bp and primer UBC 267 (5'-CCATCTTGTG-3') yielded a relatively weak polymorphic band of 1100 bp.
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