文献类型： 外文期刊

作者： Cao, Zong-Xi ¹ ; Jiao, Pei-Rong ¹ ; Huang, Yu-Mao ² ; Qin, Hong-Yang ¹ ; Kong, Liu-Wu ¹ ; Pan, Quan-Hui ¹ ; He, Yi-Min ¹ ;

作者机构： 1.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

2.Hainan Acad Agr Sci, Inst Anim Husb & Vet Res, Haikou 571100, Peoples R China

关键词： PRRSV;ORF5;genetic diversity analysis;South China;subgenotype

期刊名称：ACTA VETERINARIA HUNGARICA （ 影响因子：0.955； 五年影响因子：1.164 ）

ISSN： 0236-6290

年卷期： 2012 年 60 卷 1 期

页码：

收录情况： SCI

摘要： To understand the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in South China, we collected 231 clinical samples from pigs with suspected PRRSV infection in Guangdong between 2007 and 2009. We found that 74 of 231 samples were positive by RT-PCR. The PCR products of the ORF5 gene of 35 isolates from different farms were sequenced and their DNA sequences were compared to 23 other PRRSV isolates in the GenBank. We found that the nucleotide similarity among all South China isolates ranged from 87.6% to 100%, and all belonged to the North American genotype. Most of them were classified into subgenotype I, but the rest mapped to subgenotypes III, V or VI. Those in subgenotypes I and III were found to be highly variable in the primary neutralising epitope (PNE) with a specific amino acid mutation (F39/L39 -> I39), and a few isolates in subgenotypes I and III isolates also had a mutation at L41 (L41 -> S41). PRRSV isolates in subgenotypes III, V and VI had less potential glycosylation sites than those in subgenotype I. Our data contribute to the understanding of molecular variation of PRRSV in South China.