文献类型： 外文期刊

作者： Wang, Xilong ¹ ; Ou, Jiangtao ² ; Huang, Liguang ² ; Nishihara, Masugi ² ; Li, Junyou ³ ; Manabe, Noboru; Zhang, Yu ¹ ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China; Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Peoples R China; Univ Tokyo, Dept Vet Physiol, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan; Univ Tokyo, Grad Sch Agr & Life Sci, Anim Resource Sci Ctr, Ibaraki 3190206, Japan

3.China Agr U

关键词： gene scanning;genetic polymorphism;microsatellite;multiplex polymerase chain reaction (PCR);Wuzhishan miniature pig

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN： 0916-8818

年卷期： 2006 年 52 卷 5 期

页码：

收录情况： SCI

摘要： The Wuzhishan miniature pig originates from Hainan Island, P. R. China, and is considered useful for medical and veterinary research due to its small size; a mature adult weights less than 25 kg. To reveal the genetic characteristics of this breed, the genetic polymorphism in 4 inbred strains was estimated using 30 microsatellite genes, multiplex polymerase chain reaction, and gene scanning. The average number of alleles at the 30 marker loci was 8.300, 4.533, 6.700, and 7.433, respectively. The polymorphism information content was 0.632, 0.507, 0.653, and 0.691, respectively. The average homozygosity in these strains was 0.537, 0.546, 0.549, and 0.520, respectively. These results indicate a relatively high degree of heterozygosity, perhaps becaquse these strains were inbred for 3 generations. Therefore, a long-term inbred breeding program should be conducted for Wuzhishan pigs to establish an experimental model.