您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cryopreservation and Cryotolerance Mechanism in Zygotic Embryo and Embryogenic Callus of Oil Palm

文献类型： 外文期刊

作者： Wei, Qiuen 1 ; Shi, Peng 2 ; Khan, Faiza Shafique 3 ; Htwe, Yin Min 3 ; Zhang, Dapeng 2 ; Li, Zhiying 2 ; Wei, Xueke 2 ; Yu, Qun 3 ; Zhou, Kaibing 1 ; Wang, Yong 2 ;

作者机构： 1.Hainan Univ, Sch Hort, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Coconut Res Inst, Hainan Key Lab Trop Oil Crops Biol, Wenchang 571339, Peoples R China

3.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya 572000, Peoples R China

4.Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China

关键词： cryopreservation; cryotolerance; micropropagation; genetic stability; transcriptional changes

期刊名称：FORESTS （ 影响因子：2.9； 五年影响因子：3.0 ）

ISSN：

年卷期： 2023 年 14 卷 5 期

页码：

收录情况： SCI

摘要： Oil palm (Elaeis guineensis) is the highest oil-yielding commercially grown perennial tree. Oil palm germplasm conservation and in vitro clonal propagation strengthened the world's efforts to ensure future food security. Cryopreservation provides long-term storage for germplasm. The storage of plant material at cryogenic temperatures (-196 degrees C) following dehydration causes cryoinjury. The cryotolerance mechanism has rarely been studied in oil palm zygotic embryos (ZE) and embryogenic calli (EC). A simple and effective cryopreservation method was established for ZE. ZE surrounded by endosperm was air-dried for 3 days without any complicated chemical pre-treatments before cryopreservation, while the viability rate and following germination rate could reach up to 96.67% and 90.88%, respectively. As for EC, the preferred method could be pre-culture in liquid MS medium with 0.3 M sucrose for 12 h and PVS2 treatment for 5 min prior to cryopreservation, and the viability rate reached 68.33%. SSR markers were used to verify the genetic stability after cryopreservation. In addition, changes in enzyme activities (CAT, POD, and SOD) showed a consistent trend with H2O2 production among ZE samples, indicating that these antioxidants were involved in ROS scavenging. Furthermore, differently expressed genes (DEGs) related to ROS, osmotic, and cold stress responses were selected for correlation network analysis. Most genes involved in ROS production (RBOH, PAO, and PRX) and ROS scavenging (APX, PER, SOD, CAT, GPX, and AOX) showed higher expression levels in EC, suggesting that EC was more sensitive to oxidative stress than ZE. The cryotolerance mechanism was further summarized accordingly. These results contributed to cryopreservation methods and provided a better understanding of cryotolerance in oil palm.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台