文献类型： 外文期刊

作者： Yi Qing ¹ ; Miao Hong ² ; Wu Yong-Ning ¹ ;

作者机构： 1.Wuhan Polytech Univ, Sch Food Sci & Engn, Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430000, Peoples R China

2.China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China

关键词： Food;Chloropropanols;Online gel permeation chromatography-gas chromatography-triple quadrupole mass spectrometry;Matrix solid-supported liquid-liquid extraction;Derivatization-free

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2016 年 44 卷 5 期

页码：

收录情况： SCI

摘要： A derivatization-free method for chloropropanols determination in food using online gel permeation chromatography-gas chromatography. triple quadrupole mass spectrometry (Online GPC-GC-MS/MS) with free of derivatization was established. The target compounds were 3-monochloropropane-1,2-diol (3-MCPD), 2-monochloro-propane-1,3-diol (2-MCPD), 1,3-dichloropropan-2-ol (1,3-DCP) and 2,3-dichloropropan-1-ol (2,3-DCP). Samples were spiked with isotope internal standards, and extracted by matrix solid-supported liquid. liquid extraction on the ExtrelutTM NT absorbent. Hexane was added to wash away the apolar matrix interferences and then ethyl acetate was used to extract the target compounds. The concentrated extracts were directly injected into Online GPC-GC-MS/MS. The good linear relationships for the four types of analytes were obtained in the concentration range of 0. 005-1. 000 mg/L with correlation coefficients not less than 0. 999. The limits of detection and quantitation for chloropropanols in the food samples were in the ranges of 0. 0020. 005 mg/kg, and 0. 005-0. 01 mg/kg respectively. The recoveries and the relative standard deviations (RSD, n = 6) for the spiked blank samples at the levels of 0. 02, 0. 1, 0. 5 mg/kg were in the ranges of 94. 8% -106. 3% (2. 2% -10. 3%), 91. 8% -108. 8% (2. 1% -10. 6%) and 83. 1% -109. 4% (1. 3% 9. 4%), respectively. The established method was applied to the samples of soy sauce, hydrolyzed vegetables protein solution and powder, cooking wine, chicken powder, bread and cake, and satisfactory results were acquired.