Checking transformation efficiency for different Lagenaria siceraria genotypes by using seed germination pouches as a growth carrier
文献类型： 外文期刊
作者： Wang, Ying 1 ; Huang, Lijuan 1 ; Li, Yanwei 1 ; Feng, Zishan 1 ; Mu, Zihan 1 ; Wang, Jian 1 ; Wu, Xinyi 1 ; Wang, Baogen 1 ; Lu, Zhongfu 1 ; Li, Guojing 1 ; Wu, Xiaohua 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
关键词： Bottle gourd; Agrobacterium rhizogenes; Genetic transformation; Hairy root; Seed germination pouch
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）
ISSN： 0167-6857
年卷期：
页码：
收录情况： SCI
摘要： Bottle gourd [Lagenaria siceraria (Mol.) Standl.] is one of the most common horticultural and medicinal crops. Genetic transformation is a rate-limiting step in unraveling the function of genes and molecular breeding in this non-model crop. In this study, we established an optimized protocol for transformation using a seed germination pouch as a growth carrier and developed bottle gourd composite plants. Overground explants were inoculated with Agrobacterium rhizogenes strain R1000, which harbored a binary vector pBI121 carrying the GUS reporter gene. The integration of GUS into transgenic roots was confirmed via reverse transcription-polymerase chain reaction and histochemical staining. Furthermore, we screened for desirable genotypes from 14 bottle gourd germplasm collections based on the number of induced roots, transformation efficiency, and induction rate, and "Changguang" (transformation efficiency, approximately 36.8%) was selected as the genotype suitable for hairy root induction in bottle gourd. The optimized protocol presented here is a rapid, suitable, and efficient tool for studying the function of genes and root biology of bottle gourd in the future. Key message This study developed an optimized Agrobacterium rhizogenes-mediated transformation protocol for developing bottle gourd composite plants and screened the desirable genotype.
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