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Molecular cloning, expression and bioactivity of B cell activating factor (BAFF) in African ostrich

文献类型： 外文期刊

作者： Yang, Keli 1 ; Xiao, Ke 1 ; Huang, Haibo 1 ; Lu, Shun 1 ; Zhong, Juming 1 ; Ansari, Abdur Rahman 1 ; Khaliq, Haseeb 1 ; S 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Hubei, Peoples R China

3.Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA

关键词： Ostrich (Struthio camelus);BAFF;Expression;B cell survival

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：4.932； 五年影响因子：4.624 ）

ISSN： 1567-5769

年卷期： 2015 年 28 卷 1 期

页码：

收录情况： SCI

摘要： B cell activating factor (BAFF), which belongs to the tumor necrosis factor (TNF) family, is testified to play a critical role in B cell survival, proliferation, maturation and inununoglobulin secretion. In the present study, the cDNA of open reading frame (ORE) in African ostrich (Struthio camelus) BAFF (designated OsBAFF) was cloned by reverse transcription-PCR (RT-PCR). The OsBAFF gene encodes a 288-amino acid protein containing a predicted transmembrane domain and a putative furin protease cleavage site like BAFFs from chicken (cBAFF), quail (qBAFF), duck (dBAFF), goose (gBAFF) and dove (doBAFF). RT-PCR.analysis showed that the OsBAFF gene is strongly expressed in the bursa of Fabricius, thymus, spleen, and bone marrow. The soluble OsBAFF had been cloned into pET28a. SDS-PAGE and Western blotting analysis confirmed that the soluble fusion protein His-OsBAFF was efficiently expressed in Escherichia coli Rosset (DE3). In vitro, purified OsBAFF was not only able to promote the survival of African ostrich bursal lymphocytes, but also able to co-stimulate proliferation of mouse splenic B cells. The expression of OsBAFF in lymphocyte cells was higher than the control after LPS stimulation. These findings indicated that OsBAFF plays an important role in survival and proliferation of African ostrich bursal lymphocytes, which may provide valuable information for research into the immune system of African ostrich and OsBAFF may serve as a potential immunologic factor for enhancing immunological efficacy in African ostrich and any other birds. (C) 2015 Elsevier B.V. All rights reserved.

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