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Rapid, simple and selective determination of 2,4-dinitrophenol by molecularly imprinted spin column extraction coupled with fluorescence detection

文献类型： 外文期刊

作者： Fang, Min 1 ; Lei, Fang 1 ; Zhou, Jia 1 ; Wu, Yong-Ning 3 ; Gong, Zhi-Yong 1 ;

作者机构： 1.Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China

2.Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430023, Peoples R China

3.China Natl Ctr Food Safety Risk Assessment, Beijing 100050, Peoples R China

关键词： Molecularly imprinted solid phase extraction;Indirect fluorescence detection;Spin column;2,4-Dinitrophenol

期刊名称：CHINESE CHEMICAL LETTERS （ 影响因子：6.779； 五年影响因子：4.558 ）

ISSN： 1001-8417

年卷期： 2014 年 25 卷 11 期

页码：

收录情况： SCI

摘要： A rapid, simple and selective method based on molecularly imprinted, spin column extraction coupled with fluorescence detection was successfully established for the determination of 2,4-dinitrophenol in serum samples. The 2,4-dinitrophenol imprinted polymers exhibited highly selective recognition for the template molecule and the maximum adsorption capacity was 138.9 mg/g. The results indicated that when water is used as the loading solution, only 2,4-dinitrophenol could be adsorbed on the spin column without the remaining structural analogs (2-nitrophenol, 4-nitrophenol and phenol). After eluting with acetonitrile/acetic acid (9/1, v/v), 2,4-dinitrophenol in serum samples could be determined by using the fluorescence spectrometer, based on the fluorescence enhancement of fluorescein by the template molecule. Under the optimal conditions, the spiked recovery ranged from 95.8% to 103.4% and the detection limit was 1 nmol/L. The results confirmed the reliability and practicality of the protocol and revealed a good perspective of this method for biological sample analysis. (C) 2014 Zhi-Yong Gong. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

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