Development and Application of SYBR Green I Real-Time Fluorescence PCR for Detection of Porcine Epidemic Diarrhea Virus
文献类型： 外文期刊
作者： Guo, Rui 1 ; Tian, Yongxiang 1 ; Zhou, Danna 1 ; Duan, Zhengying 1 ; Yang, Keli 1 ; Liu, Zewen 1 ; Yuan, Fangyan 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Hunan, Peoples R China
期刊名称：2014 INTERNATIONAL CONFERENCE ON BIOLOGICAL ENGINEERING AND BIOMEDICAL (BEAB 2014)
ISSN：
年卷期： 2014 年
页码：
收录情况： SCI
摘要： With the conservative gene sequence ORF3 of porcine epidemic diarrhea virus (PEDV) as reference, a pair of specific PCR primers were designed and optimized. Then SYBR Green I real-time fluorescence PCR was developed for rapid quantitative detection of PEDV. This method has a sensibility 100 times higher than RT-PCR and avoids the high pollution brought by conventional PCR electrophoresis. Therefore, with high speediness, sensibility, specificity, repeatability, and quantifying ability, this method can be used in rapid detection of PEDV in pig farms.
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