Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy
文献类型： 外文期刊
作者： Hu, G. J. 1 ; Hong, N. 1 ; Wang, L. P. 1 ; Hu, H. J. 3 ; Wang, G. P. 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China
3.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430209, Hubei, Peoples R China
关键词： Pear;In vitro culture;Thermotherapy;Chemotherapy;Virus elimination
期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）
ISSN： 0261-2194
年卷期： 2012 年 37 卷
页码：
收录情况： SCI
摘要： In vitro plants of sand pear (Pyrus pyrifolia cv. Jinshui no. 2), which were infected by Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV), were treated by chemotherapy with ribavirin, thermotherapy at 35 +/- 0.5 degrees C and the combined application of both methods. Results showed that chemotherapy with ribavirin at 15-25 mu g/ml for 5-30 days could enhance the growth and proliferation of in vitro pear plants. Chemotherapy combined with thermotherapy could greatly improve the efficiency of virus eradication as it was compared with the separated application of both methods. A high virus eradication efficiency of 100% was achieved by chemotherapy of ribavirin at 25 mu g/ml combined with thermotherapy at 35 degrees C for 40 days, followed by culturing of 0.5-1 mm long meristem-tips. The efficacy of ribavirin for the elimination of both ASGV and ACLSV had no significant difference. (C) 2012 Elsevier Ltd. All rights reserved.
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