文献类型： 外文期刊

作者： Wang, X. Q. ¹ ; Feng, C. H. ¹ ; Lin, Z. X. ¹ ; Zhang, X. L. ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Inst Ind Crops, Wuhan, Hubei, Peoples R China

关键词： Sea-island cotton;Genetic diversity;Mapped SSR

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2011 年 10 卷 4 期

页码：

收录情况： SCI

摘要： In order to evaluate the genetic diversity of sea-island cotton (Gossypium barbadense), 237 commonly mapped SSR markers covering the cotton genome were used to genotype 56 sea-island cotton accessions. A total of 218 polymorphic primer pairs (94.75%) amplified 361 loci, with a mean of 1.66 loci. Polymorphism information content values of the SSR primers ranged from 0.035 to 0.862, with a mean of 0.320. The highest mean polymorphism information content value for the SSR motifs was from a compound motif (0.402), and for the chromosomes it was Chr10 (0.589); the highest ratio of polymorphic primers in Xinjiang accessions was from Chr21 (83.33%). Genetic diversity was high in Xinjiang accessions. AMOVA showed that variation was 8 and 92% among populations and within populations, respectively. The 56 sea-island accessions were divided into three groups in the UPGMA dendrogram: Xinhai5 was in the first group; accessions from Xinjiang, except the five main ones, were in the second group, and the other 34 accessions were in the third group. Accessions from the former Soviet Union and Xinjiang main accessions were closely related. Both PCA and UPGMA confirmed that Xinhai5 was distinct from the other accessions, and accessions from Xinjiang were in an independent group. Given the differences between principal components analysis and UPGMA results, it is necessary to combine molecular markers and pedigree information so that genetic diversity can be objectively analyzed.