文献类型： 外文期刊

作者： Gao Hong ¹ ; Shi Defang ¹ ; Guo Peng ¹ ; Yang De ¹ ; Zhou Ming ¹ ;

作者机构： 1.Hubei Acad Agr Sci, Res Inst Agr Prod Proc & Nucl Agr Technol, Wuhan, Peoples R China

关键词： Agaricus blazei Murrill;ergosterol;polysaccharides;bistage control fermentation

期刊名称：2011 3RD WORLD CONGRESS IN APPLIED COMPUTING, COMPUTER SCIENCE, AND COMPUTER ENGINEERING (ACC 2011), VOL 3

ISSN： 2160-0708

年卷期： 2011 年 3 卷

页码：

收录情况： SCI

摘要： On the basis of previous studies, the fermentation production of ergosterol and polysaccharides from Agaricus blazei Murrill was further optimized in a 5L mechanically stirred-fermenter. The bi-stage control technique, that is, shifting the pH and culture temperature from 4.43 and 26 degrees C to 5.43 and 22 degrees C respectively at 48 hr, was introduced to improve mycelia growth and exopolysaccharide production. The other parameters were set at 309 rev/min of agitation speed, 1 vvm (3.0 L/min) of aeration rate, 8%of inoculation density and 0.015 Mpa of tank pressure. The yield under the optimal condition were 11.52 g/L of dry mycelia, 107 mg/L of ergosterol, 260 mg/L intracellular polysaccharide and 298 mg/L exopolysaccharide, which were 7.9%, 44.6%, 21.5% and 3.7% higher than the pH-uncontrolled fermentation at constant temperature, respectively.