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A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish

文献类型： 外文期刊

作者： Wang, Zhi Wei 1 ; Zhang, Li Jun 1 ; Chen, Jie 1 ; Xiang, Chang Ping 2 ; Mei, Shi Yong 4 ; Zhou, Yuan 1 ; Wang, Ting 1 ;

作者机构： 1.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430074, Peoples R China

2.Huazhong Agr Univ, Key Lab Minist Educ Hort Plant Biol, Wuhan 430070, Peoples R China

3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

4.Hubei Acad Agr Sci, Wuhan 430064, Peoples R China

关键词： Cosegregate;Cytoplasmic male sterility;Fertility restorer;Intergenic recombination;Radish

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2010 年 25 卷 2 期

页码：

收录情况： SCI

摘要： In this work, we have identified a chimeric pentatricopeptide repeat (PPR)-encoding gene cosegregating with the fertility restorer phenotype for cytoplasmic male sterility (CMS) in radish. We have constructed a CMS-Rf system consisting of sterile line '9802A2', maintainer line '9802B2' and restorer line '2007H'. F(2) segregating population analysis indicated that male fertility is restored by a single dominant gene in the CMS-Rf system described above. A PPR gene named Rfoc was found in the restorer line '2007H'. It cosegregated with the fertility restorer in the F(2) segregating population which is composed of 613 fertile plants and 187 sterile plants. The Rfoc gene encodes a predicted protein 687 amino acids in length, comprising 16 PPR domains and with a putative mitochondrial targeting signal. Sequence alignment showed that recombination between the 5' region of Rfob (EU163282) and the 3' region of PPR24 (AY285675) resulted in Rfoc, indicating a recent unequal crossing-over event between Rfo and PPR24 loci at a distance of 5.5 kb. The sterile line '9802A2' contains the rfob gene. In the F(2) population, Rfoc and rfob were observed to fit a segregation ratio 1:2:1 showing that Rfoc was allelic to Rfo. Previously we have reported that a fertile line '2006H', which carries the recessive rfob gene, is able to restore the male fertility of CMS line '9802A1' (Wang et al. in Theor Appl Genet 117:313-320, 2008). However, here when conducting a cross between the fertile line '2006H' and CMS line '9802A2, the resulting plants were male sterile, which shows that sterile line '9802A2' possesses a different nuclear background compared to '9802A1'. Based on these results, the genetic model of fertility restoration for radish CMS is also discussed.

  • 相关文献

[1]Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance. Wang, Zhi Wei,Wang, Chuan,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Chuan,Mei, Shi Yong,Xiang, Chang Ping. 2013

[2]Development of PCR-based markers linked to a restorer gene for cytoplasmic male sterility in radish (Raphanus sativus L.). Wang, Zhiwei,Xiang, Changping,Mei, Shiyong. 2006

[3]Effect of N Management on Root Yield and N Uptake of Radishes in Southern China. Yuan, Wei-Ling,Deng, Xiao-hui,Gan, Cai-xia,Cui, Lei,Wang, Qing-fang,Yuan, Shang-yong.

[4]An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.). Wang, Zhi Wei,Wang, Chuan De,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Chuan De,Mei, Shi Yong.

[5]Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.). Wang, Zhi-Wei,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Zhi-Wei,Xiang, Chang Ping,Liu, Hai Zhou,Mei, Shi Yong.

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