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Authentication of Panax ginseng from its Adulterants by PCR-RFLP and ARMS

文献类型： 外文期刊

作者： Diao, Ying 1 ; Lin, Xian-Ming 3 ; Liao, Chao-Lin 3 ; Tang, Chun-Zi 3 ; Chen, Zhong-Jian 4 ; Hu, Zhong-Li 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China

2.Chongqing Univ Arts & Sci, Coll Life Sci & Technol, Chongqing, Peoples R China

3.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi, Peoples R China

4.Wenshan Sanqi Res Inst, Wenshan, Peoples R China

关键词： Panax ginseng;Araliaceae;authentication;PCR-RFLP;5S rDNA NTS;ARMS

期刊名称：PLANTA MEDICA （ 影响因子：3.352； 五年影响因子：3.4 ）

ISSN： 0032-0943

年卷期： 2009 年 75 卷 5 期

页码：

收录情况： SCI

摘要： As a widely used and expensive herbal medicine, Panax ginseng has many adulterants in the commercial market. PCR-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) based on 5S rDNA sequence analysis were applied to identify two common adulterants of P. ginseng. The sizes of 5S rRNA gene non-transcribed spacers (NTS) sequences in R ginseng and its adulterants were determined, ranging from 143 to 424 bp. The PCR product of P. ginseng only could be digested among the tested specimens because of its specific Spel restriction site found in the 5S rDNA sequence. In addition, P. ginseng was successfully identified from compound medicinal preparations and from the Single-Taste medicines. These results suggest that the methods are able to authenticate P. ginseng.

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