Identification of Self-Incompatibility Genotypes in Some Sand Pears (Pyrus pyrifolia Nakai) by PCR-RFLP Analysis
文献类型： 外文期刊
作者： Gu Qing-qing 1 ; Zhang Qing-lin 1 ; Hu Hong-jue 3 ; Chen Qi-liang 3 ; Luo Zheng-rong 1 ;
作者机构： 1.Huazhong Agr Univ, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China
2.Jiangxi Agr Univ, Dept Hort, Nanchang 330045, Peoples R China
3.Hubei Acad Agr Sci, Fruit Tree & Tea Res Inst, Wuhan 430209, Peoples R China
关键词： Pyrus pyrifolia Nakai;self-incompatibility genotype;S-allele;S-RNase;PCR-RFLP;crossing experiment
期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 2013影响因子：0.82； 五年影响因子：0.997 ）
ISSN： 1671-2927
年卷期： 2009 年 8 卷 2 期
收录情况： SCI
摘要： The identification of self-incompatibility genotype (S-genotype) will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-RNase sequencing. The S-genotypes of these cultivars were determined as follows: Huali 1 S(1)S(3), Shounan S(1)S(3), Xizilu S(1)S(4), Qingxiang S(3)S(7), Sanhua S(2)S(7), Huangmi (Imamuranatsu) S(1)S(6), Huali 2 S(3)S(4), Baozhuli S(7)S(33), Cangxixueli S(5)S(15). S-RNase alleles (S(1)to S(9)) in sand pear could be identified effectively by PCR-RFLP analysis.
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