湖北省农业科学院机构知识库

Identification of integration of foreign gene in transgenic pigs

收藏
分享
全文链接

文献类型：外文期刊

作者： Liu, GS ¹ ; Chen, QX ² ; Jing, W ³ ;

作者机构： 1.ACAD SINICA,INST DEV BIOL,BEIJING 100080,PEOPLES R CHINA

2.HUBEI ACAD AGR SCI,INST ANIM HUSB,WUHAN 430209,PEOPLES R CHINA

3.AGR UNIV CHINA,INST BIOTECHNOL,BEIJING 100094,PEOPLES R CHINA

关键词： porcine growth hormone gene;PCR;DNA blotting;transgenic pig

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （影响因子：0.351；五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 1996 年 23 卷 3 期

页码：

收录情况： SCI

摘要： Foreign porcine growth hormone genes were introduced into Hubei white pigs. In order to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection, The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration.

相关文献

[1]Rapid and Sensitive Detection of sFAT-1 Transgenic Pigs by Visual Loop-Mediated Isothermal Amplification. Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Zhou, Rong,Zheng, Xinmin,Ren, Hongyan.

[2]Safety assessment of sFat-1 transgenic pigs by detecting their co-habitant microbe in intestinal tract. Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Zheng, Xinmin,Chen, Hongxing.

[3]Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs. Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Zheng, X. M.,Hou, J..

[4]猪胞内劳森氏菌lscN基因的克隆及序列分析. 张敏,尹会方,屠晶. 2017

[5]分子技术对表达GFP转基因胚胎的再检测. 肖红卫,郑新民,李莉,陈思怀,张涌,魏庆信. 2006

[6]PCR扩增猪ESR基因一个外显子DNA片段的研究. 徐子清,梅书棋,华文君,吴金英. 1998

[7]福建省某公猪站布氏杆菌病的诊断. 孙艳发,张敏,戴爱玲,李晓华,杨小燕,屠晶. 2015

[8]用聚合酶链式反应技术鉴定杂交稻种子纯度的初步研究. 戚华雄,杨蜀兰,王斌,卢兴桂. 1999

[9]一种高效提取桑树根围土壤微生物总DNA的方法. 黄淑君,胡兴明,于翠,邓文,叶楚华. 2012

[10]猪巴氏杆菌病的病原检测及耐药性分析. 卢琴,李明波,彭先文,宋忠旭,雷彬,梅书棋. 2015

[11]猪伪狂犬病与猪圆环病毒病混合感染的PCR诊断. 卢琴,李明波,彭先文,宋忠旭,梅书棋. 2015

Identification of integration of foreign gene in transgenic pigs

作者其他论文更多>>

意见箱

Identification of integration of foreign gene in transgenic pigs

作者其他论文 更多>>

意 见 箱

作者其他论文更多>>

意见箱