文献类型： 外文期刊

作者： Zhang, Jinying ¹ ; Li, Runbo ¹ ; Luo, Ruijie ² ; Zhang, Qiang ³ ; Zhao, Zimo ¹ ; Xu, Haishen ¹ ; Zhi, Minglei ¹ ; Jiang, Wenjie ⁴ ; Wang, Meng ¹ ; Chen, Xinze ¹ ; Feng, Zhiqiang ¹ ; Wang, Yingjie ¹ ; Yang, Yuhan ¹ ; He, Pengcheng ¹ ; Su, Hanyue ² ; Chen, Tianzhi ¹ ; Wang, Shunxin ¹ ; Yao, Yixuan ¹ ; Yang, Jinghui ² ; Zhao, Fan ² ; Li, Tingting ⁵ ; Wang, Haitao ⁵ ; Zhang, Xiaosheng ⁶ ; Zhang, Jinlong ⁶ ; Liu, Qiuyue ⁵ ; Gao, Shuai ⁷ ; Yao, Yuchang ⁴ ; Han, Jianyong ¹ ; Cao, Suying ² ;

作者机构： 1.China Agr Univ, Coll Biol Sci, State Key Lab Anim Biotech Breeding, Frontiers Sci Ctr Mol Design Breeding MOE, Beijing 100193, Peoples R China

2.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing 102206, Peoples R China

3.Xiamen Univ, Xiamen Cardiovasc Hosp, Inst Cardiovasc Dis, Sch Med, Xiamen 361006, Peoples R China

4.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150038, Peoples R China

5.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

6.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin 300380, Peoples R China

7.China Agr Univ, Coll Anim Sci & Technol, Frontiers Sci Ctr Mol Design Breeding MOE, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China

关键词： embryo development; epiblast; pluripotency; sheep; single-cell RNA seq

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Capturing different pluripotent state stem cells from epiblast in vitro helps understand embryonic development and provides invaluable cell sources for basic research and regenerative medicine. Sheep are not only one of the most important livestock species in agriculture but also serve as an ideal preclinical model for studying human disease. Single-cell transcriptome analysis of sheep preimplantation embryos from embryonic day (E) 1 to E14 is performed to investigate the pluripotency changes of epiblast and elucidate the pluripotent regulation signaling. By combination of growth factors or inhibitors of JAK/STAT3, FGF, WNT, and TGF-beta pathways in the culture medium, sheep formative and primed pluripotent stem cells (sfPSCs and spPSCs) are established respectively. The newly derived PSCs could maintain over 100 passages and differentiate into three germ layers. In addition, sfPSCs and spPSCs exhibit different molecular features, and sfPSCs have the ability of contribution to ICM and can be used as donor cells for producing cloned embryos efficiently. A cross-species comparison of early embryo development in mouse, pig and sheep illustrates the conservation of the epiblast na & iuml;ve to primed state transition process and the divergence of the developmental events time points, the specific gene expression patterns and pluripotent regulation signaling. These studies are expected to improve our understanding of mammal early embryo development and present a reference for defining pluripotency.