Gain-of-function in Arabidopsis (GAINA) for identifying functional genes in Hevea brasiliensis
文献类型: 外文期刊
作者: Cheng, Han 1 ; Gao, Jing 1 ; Cai, Haibin 1 ; Zhu, Jianshun 1 ; Huang, Huasun 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab, Minist Agr Trop Crops Physiol, Danzhou City, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Hainan, Peoples R China
关键词: Gain-of-function;Rubber tree;Arabidopsis;Full-length cDNA library;Mutation;Forward genetics
期刊名称:SPRINGERPLUS ( 影响因子:1.13; 五年影响因子:1.29 )
ISSN: 2193-1801
年卷期: 2016 年 5 卷
页码:
收录情况: SCI
摘要: Background: Forward genetics approaches are not popularly applied in non-model plants due to their complex genomes, long life cycles, backward genetic studies etc. Researchers have to adopt reverse genetic methods to characterize gene functions in non-model plants individually, the efficiency of which is usually low. Results: In this study, we report a gain-of-function in Arabidopsis (GAINA) strategy which can be used for batch identification of functional genes in a plant species. This strategy aims to obtain the gain-of-function of rubber tree genes through overexpressing transformation ready full-length cDNA libraries in Arabidopsis. An initial transformation test produced about two thousand independent transgenic Arabidopsis lines, in which multiple obvious aberrant phenotypes were observed, suggesting the gain-of-function of rubber tree genes. The transferred genes were further isolated and identified. One gene identified to be metallothionein-like protein type 3 gene was further transferred into Arabidopsis and reproduced a similar aberrant phenotype. Conclusion: The GAINA system proves to be an efficient tool for batch identification of functional genes in Hevea brasiliensis, and also applicable in other non-model plants.
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