Profiling Ethylene-Responsive Genes Expressed in the Latex of the Mature Virgin Rubber Trees Using cDNA Microarray
文献类型： 外文期刊
作者： Nie, Zhiyi 1 ; Kang, Guijuan 1 ; Duan, Cuifang 1 ; Li, Yu 1 ; Dai, Longjun 1 ; Zeng, Rizhong 1 ;
作者机构： 1.CATAS, Key Lab Biol & Genet Resources Rubber Tree, Minist Agr, Rubber Res Inst, Danzhou, Hainan, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2016 年 11 卷 3 期
页码：
收录情况： SCI
摘要： Ethylene is commonly used as a latex stimulant of Hevea brasiliensis by application of ethephon (chloro-2-ethylphosphonic acid); however, the molecular mechanism by which ethylene increases latex production is not clear. To better understand the effects of ethylene stimulation on the laticiferous cells of rubber trees, a latex expressed sequence tag (EST)based complementary DNA microarray containing 2,973 unique genes (probes) was first developed and used to analyze the gene expression changes in the latex of the mature virgin rubber trees after ethephon treatment at three different time-points: 8, 24 and 48 h. Transcript levels of 163 genes were significantly altered with fold-change values >= 2 or <=-2 (q-value < 0.05) in ethephon-treated rubber trees compared with control trees. Of the 163 genes, 92 were up-regulated and 71 down-regulated. The microarray results were further confirmed using real-time quantitative reverse transcript-PCR for 20 selected genes. The 163 ethylene-responsive genes were involved in several biological processes including organic substance metabolism, cellular metabolism, primary metabolism, biosynthetic process, cellular response to stimulus and stress. The presented data suggest that the laticifer water circulation, production and scavenging of reactive oxygen species, sugar metabolism, and assembly and depolymerization of the latex actin cytoskeleton might play important roles in ethylene-induced increase of latex production. The results may provide useful insights into understanding the molecular mechanism underlying the effect of ethylene on latex metabolism of H. brasiliensis.
- 相关文献
作者其他论文 更多>>
-
Molecular cloning, expression profiles, characterization and function of a novel arginine decarboxylase gene (HbADC2) in Hevea brasiliensis
作者：Deng, Zhi;Hou, Lanfei;Dai, Longjun;Li, Dejun;Deng, Zhi;Hou, Lanfei;Dai, Longjun;Li, Dejun;Deng, Zhi;Deng, Zhi;Hou, Lanfei;Dai, Longjun;Li, Dejun;Hou, Lanfei;Men, Zhonghua;Xia, Zhihui;Li, Yao;Li, Dejun
关键词：Arginine decarboxylase (ADC); Rubber tree; Polyamine; MV-induced oxidative stress; Putrescine (Put)
-
In situ mass spectrometry imaging reveals pesticide residues and key metabolic pathways throughout the entire cowpea growth process
作者：Jiang, Shufan;Lin, Jingling;Zhang, Rui;Wu, Qiong;Li, Hongxing;Zhang, Qun;Wang, Mingyue;Xie, Defang;Zhang, Yue;Han, Bingjun;Jiang, Shufan;Lin, Jingling;Zhang, Rui;Wu, Qiong;Li, Hongxing;Zhang, Qun;Wang, Mingyue;Xie, Defang;Zhang, Yue;Han, Bingjun;Jiang, Shufan;Lin, Jingling;Zhang, Rui;Wu, Qiong;Li, Hongxing;Zhang, Qun;Wang, Mingyue;Xie, Defang;Zhang, Yue;Han, Bingjun;Jiang, Shufan;Lin, Jingling;Zhang, Rui;Wu, Qiong;Li, Hongxing;Zhang, Qun;Wang, Mingyue;Xie, Defang;Zhang, Yue;Han, Bingjun;Jiang, Shufan;Zhang, Xinzhong;Jiang, Shufan;Dai, Longjun
关键词：Cowpea; Pesticide residue; In situ mass spectrometry imaging; Metabolism; Growth process
-
Taxonomic and phylogenetic contributions to Diatrypaceae from southeastern Tibet in China
作者：Ma, Hai-Xia;Yang, Zhan-En;Song, Zi-Kun;Qu, Zhi;Yang, Zhan-En;Song, Zi-Kun;Li, Yu;Zhu, An-Hong
关键词：Ascomycota; Diatrypaceous fungi; multigene phylogeny; taxonomy; wood-decaying fungi; Xylariales
-
Genome-Wide Identification and Expression Analysis of the R2R3-MYB Gene Family in Rubber Trees
作者：Liu, Mingyang;Yang, Hong;Fan, Songle;Guo, Bingbing;Dai, Longjun;Wang, Lifeng;Wang, Meng;Wang, Meng
关键词：Hevea brasiliensis Muell; Arg; HbMYB; powdery mildew; ethylene; phylogenetic analysis
-
Polysaccharides of Floccularia luteovirens Alleviate Oxidative Damage and Inflammatory Parameters of Diabetic Nephropathy in db/db Mice
作者：Wang, Huan;Yang, Yang;Li, Changtian;Li, Dan;Li, Yu;Wang, Huan;Wang, Shumin;Chen, Changbao;Wan, Xilin;Yang, Yang
关键词：Floccularia luteovirens; diabetic nephropathy; fungal polysaccharide; oxidative stress; inflammation
-
Visualization and metabolome for the migration and distribution behavior of pesticides residue in after-ripening of banana
作者：Wang, Meiran;Tian, Qiaoxia;Li, Hongxing;Wang, Mingyue;Han, Bingjun;Chen, Juncheng;Wang, Meiran;Tian, Qiaoxia;Li, Hongxing;Wang, Mingyue;Han, Bingjun;Chen, Juncheng;Wang, Meiran;Tian, Qiaoxia;Li, Hongxing;Wang, Mingyue;Han, Bingjun;Chen, Juncheng;Wang, Meiran;Tian, Qiaoxia;Li, Hongxing;Wang, Mingyue;Han, Bingjun;Chen, Juncheng;Wang, Meiran;Tian, Qiaoxia;Chen, Juncheng;Dai, Longjun;Huang, Huaping;Wan, Yi;Zhang, Yunuo
关键词：Pesticide; Behavior; Migration and distribution; Visualization; Metabolome
-
Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness
作者：Nie, Zhiyi;Kang, Guijuan;Yan, Dong;Qin, Huaide;Zeng, Rizhong;Nie, Zhiyi;Kang, Guijuan;Qin, Huaide;Zeng, Rizhong;Nie, Zhiyi;Kang, Guijuan;Qin, Huaide;Zeng, Rizhong;Yang, Lifu
关键词：Hevea brasiliensis; rubber biosynthesis; farnesyl diphosphate synthase; tapping panel dryness; transcriptome sequencing; iTRAQ; protein-protein interaction; MYB transcription factor
